smart CRP Test

Summary

C-reactive Protein (CRP) is the best known among the acute-phase proteins, a group of proteins whose concentration increases in blood as a response to inflammatory disorders. It increases already after 6 hours in acute inflammatory processes associated with bacterial infections, post operative conditions or tissue damage.

Methode-Measurement Range:

Immunturbidimetric Assay:
Serum/Plasma: 0,5-120mg/l for smart546 and 1,0-120mg/l for smart700/340 and smart700
Whole blood: 2,0-240mg/l for smart546, smart700/340 and smart700

Principle:

Kinetic determination of the concentration of CRP by photometric measurement at 546nm or 700nm of antigen-antibody reaction between antibodies to human CRP bound to polystyrene particles and CRP present in the sample.

CRP Test Procedure

Test kit preparation: Allow single test min. 10 minutes to warm to room temperature by placing the test into the test kit rack. Put test kit package back into refrigerator.

1. Sample collection

Wholeblood from fingertip

smart CRP with integrated capillary blood collection from fingertip
1a) Aspirate 5µl whole blood from finger tip

Serum or venous blood from EDTA tube

smart CRP with integrated capillary blood or serum collection from slide
1b) Aspirate 5µl EDTA wholeblood or serum from slide

 

2. Test application

Insert RFID card into smart analyser
Insert RFID card, analyser is reading:
  • calibration curve
  • test procedure
  • expiry date
  • lot number
Apply ERS cap onto ERS cuvette
Apply ERS cap firmly onto ERS cuvette
Shake ERS cartridge
Shake ERS cartridge 3-4x gently
Insert ERS cartridge into smart analyser
Insert ERS cartridge into smart analyser. Start automatic sample processing - Read result.

 

Test specification

  • immunoturbidimetric latex assay
  • haematocrit correction
  • precision
    • crp cv < 2.5% (serum)
    • crp cv < 6.4% (whole blood)
  • linearity
    • crp 0,5 - 120 mg/l (serum)
    • crp 2,0 - 240 mg/l (whole blood)