smart Homocysteine Test


Summary:

Homocysteine is a thiol containing Amino Acid produced by the intracellular demethylation of methionine. Total Homocysteine tHCY represents the sum of all forms of HCY including forms of oxidized, protein bound and free. Elevated level of tHCY has emerged as an important risk factor in the assessment of cardiovascular disease. Excess HCY in the blood stream may cause injuries to arterial vessels due to its irritant naturem and result in inflammation and plaque formation , which may eventually cause blockage of blood flow to the heart. Elevated tHCY levels are caused by four major factors including:

  • A. genetic deficiencies
  • B. nutritional deficiency in B vitamins such as B6 B12 and folate
  • C. renal failure for effective amino acid clearance
  • D. drug interactions such as nitricide, methotrexate and phenytoin

Elevated levels of tHCY are also linked with Alzheimer's disease and Osteoporosis

Assay principle:

Kinetic determination based on enzymatic Cycling Methode:
Serum: 2-50?mol/l (small lot specific variations possible)

Homocysteine Test Procedure


Test kit preparation: Allow single test min. 10 minutes to warm to room temperature by placing the test into the test kit rack. Put test kit package back into refrigerator.

1. Sample collection

capillary sample venous sample

take 20µl serum/plasma from a centrifuged capillary blood collection system


take 20µl serum/plasma from a primary tube


2. Test application

insert RFID card analyser is reading:
  • calibration curve
  • test procedure
  • expiry date
  • lot number
pipette sample into ERS (Eurolyser-Reagent-System) cuvette apply patented ERS cap insert ERS into smart analyser, press start - read result

Test specification


  • enzyme cycling assay
  • precision: cv < 3% (29µmol/l)
  • linearity: 2-50µmol/l
  • throughput: 6 samples/h

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