smart (high sensitive CRP) hsCRP Test

Summary

C-reactive Protein (CRP) is the best known among the acute-phase proteins, a group of proteins whose concentration increases in blood as a response to inflammatory disorders. A growing number of studies have examined that a more sensitive test, the so called hsCRP (high sensitive CRP) can predict recurrent cardiovascular disease,  stroke and death in different settings. High levels of hsCRP consistently predict recurrent coronary events in patients with unstable angina and acute myocardial infarction (heart attack). Higher hsCRP levels also are associated with lower survival rates in these patients.

Methode-Measurement Range:

Immunturbidimetric Assay:
Serum/Plasma:  0,25-40mg/l, cv < 6,0%
Whole blood:      0,50-40mg/l, cv < 9,0%

Principle:

Kinetic determination of the concentration of CRP by photometric measurement at 546nm or 700nm of antigen-antibody reaction between antibodies to human CRP bound to polystyrene particles and CRP present in the sample.

hsCRP Test Procedure

Test kit preparation: Allow single test min. 10 minutes to warm to room temperature by placing the test into the test kit rack. Put test kit package back into refrigerator.

1. Sample collection
capillary sample   venous sample

take 20µl whole blood form finger tip
or
take 20µl whole blood (edta) from a primary tube

2. Test application
insert RFID card analyser is reading:
  • calibration curve
  • test procedure
  • expiry date
  • lot number
 pipette sample into ERS (Eurolyser-Reagent-System) cuvette apply patented ERS cap insert ERS into smart analyser, press "start" - read result

Test specification


  • immunoturbidimetric latex assay
  • haematocrit correction
  • precision
  • cv < 6.0% (serum)
  • cv < 9.0% (whole blood)
  • linearity
  • 0,25 - 40 mg/l (serum)
  • 0,50 - 40 mg/l (whole blood)
  • test duration
  • serum: 100 sec / test
  • whole blood: 240 sec / test

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