Eurolyser Diagnostics - Premium Laboratory Equipment

NH3 test

Ammonia

About the parameter

Protein digestion produces ammonia within the intestine by deamination of amino acid. Ammonia reaches the liver by portal blood where it is attached to the urea (main path for intestinal ammonia within the urea). Heavy liver dysfunction (e.g. shunt, cirrhosis) can prevent the liver of synthesizing ammonia into urea.

As a result one will receive a lowered urea speculum in relation to creatinine aswell as increased blood ammonia. Ammonia and other protein metabolism (aromatic amino acids) that get out of control of the liver metabolism can affect the central nervous system (hepatoenzephale syndrome).

Overview

Sample material

The accuracy of ammonia determination is extremely dependent on sample collection. Use only fresh EDTA plasma (ammonia heparin plasma or serum must not be used). After drawing the blood immediately centrifuge the collection tube to get plasma. The plasma sample has to be analysed within 5 minutes on the instrument.
 

Method / Measurement range

Enzymatic test. Photometric measurement of the absorbance rate (kinetic) at 340nm wavelength.

10 – 1100 ┬Ámol/L (LOT dependent)
 

Test duration

This test takes approx. 8.5 minutes.
 

Package insert

More details can be found in the package insert.

Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.


Appropriate instruments

Additional information

Article numbers

VT 0160      NH3 Ammonia test kit (containing 6 tests)
VT 1600      NH3 Ammonia control kit (decision level)

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