About the parameter
Thyroxin (T4) is formed in thyroid and released into bloodstream, passing a complex regulating mechanism. Thyroxin is bound to several carrier proteins such as albumin, pre-albumin and other specific binding proteins. Thyroid diseases with abnormal hormone concentrations mainly occurs to animals with an higher age.
Increased – or decreased, respectively – concentration levels of thyroid hormones such as Thyroxin (T4) lead towards numerous organic disorders. Thyroid hyperfunction occurs mainly to cats, whereas thyroid hypofunction affects all species.
Serum or plasma (EDTA, heparin, citrat). Sample volume according to the menu settings on Eurolyser solo instrument and according to the use of a 20µl or 5µl pipette.
Method / Measurement range
The Eurolyser solo T4-vet analysis is a homogenous enzyme immunoassay. By help of 8-anilino-1-naphtalin acid sulfur (ANS) the bound T4 is seperated from its natural binding proteins. The so from blood seperated thyroxin competes with a thyroxin bound to a glucose-6-phosphat dehydrogenase to form a specific connector. GAPDH activity at 340nm is associated with the total thyroxin concentration in blood. If T4 is absent in the blood sample the enzyme activity is inhibited by binding of a specific antibody.
When using a 20 µl pipette: 0 – 8 µg/dl (0 – 104 nmol/l)
When using a 5 µl pipette: 6 – 32 µg/dl (78 – 416 nmol/l)
This Test takes approx. 6 minutes.
More details can be found in the package insert.
Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.
Find the evaluation study for the Eurolyser T4 assay here: Eurolyser T4 test evaluation
VT 0140 T4 test kit (containing 16 tests)
VT 0141 T4 test kit (containing 6 tests)
VT 1400 T4 control kit (decision level)