Eurolyser Diagnostics - Premium Laboratory Equipment

FOB Test

Faecal Occult Blood

About the parameter

The presence of hemoglobin in feces is indicative of the presence of blood as a result of bleedings associated to some pathologies of the gastrointestinal tract (called faecal occult blood, FOB). Such pathologies include colon polyps, adenomas, colorectal carcinoma, ulcerative colitis and Crohn’ disease.

Additionally, FOB is used to diagnose Colorectal cancer (CRC), the third most common form of cancer in western world. Often it causes no symptoms until it has reached a relatively advanced stage. Thus, many organizations recommend screening for the disease with FOB-testing.

However, colorectal bleeding can be sometimes intermittent and so negative results do not exclude the disease. Colonoscopy is recommended as definitive test. This method is worthless as screening test for diseases of the upper-gastrointestinal tract.



The latex particles coated with antibodies anti-human hemoglobin are agglutinated when they react with feces samples containing human hemoglobin. A photometric measurement at 700nm determines the value.

Test duration

The test takes approx. 5 minutes to finish.

Method – Analytical Range

Linearity: up to 1000 µg/l.

Linearity limit depends on the sample/reagent ratio as well as the calibrator value.

Samples with higher concentrations should be diluted 1:5 with NaCl 9 g/l and retested again.

Lower detection limit: Values < 25 µg/l give non-reproducible results.

Appropriate instruments

*NOTE: This article is supplied by Sentinel Diagnostics, Milano, Italy
contact: Dr. Franco Melziade, Sales Manager

Additional information

Package insert

More details can be found in the Sentinel FOB package insert as well as the the Sentinel FOB sample collector package insert.

Please note: All package inserts which can be found and downloaded on this homepage are for general information purposes only! All test, control and calibrator kits have to be processed solely according to the package insert which is delivered with the kit!

Article numbers

1156004 FOB (faecal occult blood) test kit (containing 32 tests)*
1156189 FOB (faecal occult blood) sample collector kit (containing 32 collectors)*

*NOTE: these articles are supplied by Sentinel Diagnostics, Milano, Italy
contact: Dr. Franco Melziade, Sales Manager

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