Eurolyser Diagnostics - Premium Laboratory Equipment

Phenobarbital test

Therapeutic drug monitoring

About the parameter

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The Eurolyser phenobarbital test aids in monitoring the phenobarbital levels in animals. Phenobarbital is administered to control seizures and epilepsy, as well as to relieve cramps. Depending on the dosage, the drug has been observed to have either a sedative or a hypnotic effect. In cats, phenobarbital is the first choice for antiepileptic treatment. In dogs, it may be administered together with potassium bromide, depending on the individual reaction of the patient to the treatment.
Blood phenobarbital concentration should be checked approximately 12 to 14 days after administration. It is recommended that the blood sample be drawn approximately two hours prior to the next scheduled dose of the drug.


Sample material

Use 20 µl serum (preferred) or lithium heparin plasma

Sample material stability:
Stored at 2 – 8 °C: 3 days
Stored at -20 °C:  60 days

Method / Measurement range

Quantitative turbidimetric test for the measurement of phenobarbital in serum and lithium heparin plasma. Latex particles coated with phenobarbital are agglutinated when mixed with phenobarbital antibody solution. When a sample containing phenobarbital is used, the agglutination reaction is partially inhibited, slowing the process of agglutination. The agglutination causes an absorbance change (546 nm), inversely dependent upon the phenobarbital contents of the patient sample.

Measurement range: 0 – 100 µg/ml
Sensitivity:                      10 µg/ml

Test duration

The test takes approximately 4 minutes.

Package insert

More details can be found in the package insert

Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.

Appropriate instruments


Test evaluation

Find the evaluation study for the Eurolyser phenobarbital assay here:
Eurolyser phenobarbital test evaluation

Additional information

Article numbers

VT 0211      Phenobarbital VET test kit (containing 6 Tests)
VT 2100      Phenobarbital VET control kit (decision level)

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