Therapeutic drug monitoring
About the parameter
The Eurolyser phenobarbital test aids in monitoring the phenobarbital levels in animals. Phenobarbital is administered to control seizures and epilepsy, as well as to relieve cramps. Depending on the dosage, the drug has been observed to have either a sedative or a hypnotic effect. In cats, phenobarbital is the first choice for antiepileptic treatment. In dogs, it may be administered together with potassium bromide, depending on the individual reaction of the patient to the treatment.
Blood phenobarbital concentration should be checked approximately 12 to 14 days after administration. It is recommended that the blood sample be drawn approximately two hours prior to the next scheduled dose of the drug.
Use 20 µl serum (preferred) or lithium heparin plasma
Sample material stability:
Stored at 2 – 8 °C: 3 days
Stored at -20 °C: 60 days
Method / Measurement range
Quantitative turbidimetric test for the measurement of phenobarbital in serum and lithium heparin plasma. Latex particles coated with phenobarbital are agglutinated when mixed with phenobarbital antibody solution. When a sample containing phenobarbital is used, the agglutination reaction is partially inhibited, slowing the process of agglutination. The agglutination causes an absorbance change (546 nm), inversely dependent upon the phenobarbital contents of the patient sample.
Measurement range: 0 – 100 µg/ml
Sensitivity: 10 µg/ml
The test takes approximately 4 minutes.
More details can be found in the package insert
Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.
Find the evaluation study for the Eurolyser phenobarbital assay here:
Eurolyser phenobarbital test evaluation
VT 0211 Phenobarbital VET test kit (containing 6 Tests)
VT 2100 Phenobarbital VET control kit (decision level)