Eurolyser Diagnostics - Premium Laboratory Equipment

T4 Test


About the parameter

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Thyroxin (T4) is formed in thyroid and released into the bloodstream, passing a complex regulating mechanism. Thyroxin is bound to several carrier proteins such as albumin, pre-albumin and other specific binding proteins. Thyroid diseases with abnormal hormone concentrations mainly effect older animals.

Increased – or decreased, respectively – concentration levels of thyroid hormones such as Thyroxin (T4) lead towards numerous organic disorders. Thyroid hyperfunction effect mainly cats, whereas thyroid hypofunction affects all species.



Sample material

Serum or plasma (EDTA, heparin, citrat). Sample volume according to the menu settings on Eurolyser solo instrument and according to the use of a 20 µl or 5 µl pipette.

Method / Measurement range

The Eurolyser solo T4 VET analysis is a homogenous enzyme immunoassay. By help of 8-anilino-1-naphtalene acid sulfur (ANS) the bound T4 is seperated from its natural binding proteins. The thyroxin seperated from blood competes with a thyroxin bound to a glucose-6-phosphat dehydrogenase to form a specific connector. GAPDH activity at 340 nm is associated with the total thyroxin concentration in blood. If T4 is absent in the blood sample the enzyme activity is inhibited by binding of a specific antibody.

Measurement range
When using a 20 µl pipette: 0.4 – 8 µg/dl (5.2 – 104 nmol/l)
When using a 5 µl pipette: 6 – 32 µg/dl (78 – 416 nmol/l)

Test duration

This Test takes approx. 6 minutes.

Package insert

More details can be found in the package insert.

Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.

Appropriate instruments


Test evaluation

Find the evaluation study for the Eurolyser T4 assay here: Eurolyser T4 test evaluation

Additional information

Article numbers

VT 0140      T4 test kit (containing 16 tests)
VT 0141      T4 test kit (containing 6 tests)
VT 1400      T4 control kit (decision level)

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