beta-Hydroxybutyrate Test
beta-Hydroxybutyrate (BHB)
About the parameter
beta-Hydroxybutyrate (BHB) is one of three main ketone bodies that the liver produces to provide energy to muscles and other tissues when glucose levels are low. In diabetic individuals, those ketone bodies can build up in the blood-stream and lead to diabetic ketoacidosis – a life-threatening condition, if untreated.
Not only is BHB produced at a higher rate than the other main ketone bodies, but also predominantly at the onset of diabetic ketoacidosis, making it a reliable early phase detection marker.
Overview
Sample material
20 µl of Li-hep plasma or serum.
Method / Measurement range
The beta-Hydroxybutyrate assay is based on the principle of enzymatic determination with beta-Hydroxybutyrate-dehydrogenase. beta-Hydroxybutyrate-dehydrogenase converts beta-Hydroxybutyrate in presence of NAD+ to acetoacetate and NADH + H+. At a wavelength of 340 nm the absorbance is proportional to the beta-Hydroxybutyrate concentration in the sample.
Measurement range
0.08 – 10 mmol/l
Test duration
This test takes approx. 10 minutes.
Package insert
More details can be found in the package insert
Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.
Appropriate instruments
 EUROLyser CUBE-VET |
 EUROLyser solo |
Evaluation
Test evaluation
Find the evaluation study for the EUROLyser beta-Hydroxybutyrate assay here: EUROLyser beta-Hydroxybutyrate test evaluation
Additional information
Article numbers
VT0310 beta-Hydroxybutyrate test kit (containing 16 tests)
VT0311 beta-Hydroxybutyrate test kit (containing 6 tests)
VT3100 beta-Hydroxybutyrate control kit (decision level)