Fructosamine test
Gold standard
About the parameter
Fructosamine is an ideal parameter for the monitoring of diabetic animals. Fructosamine arises by the binding of glucose to proteins. In dogs glucose has a stronger affinity to albumins, and globulins in cats. Because of the half-life time of plasma proteins a single measurement of fructosamine indicates the average glucose concentration over the previous 1 to 2 weeks.
Individual measurements of fructosamine should be interpreted in view of the clinical symptoms of diabetes, the body weight and the blood glucose concentration. The general rule is, the closer the fructosamine concentration to the reference range for healthy dogs and cats, the better the glycemic control.
Eurolyser’s “Gold standard fructosamine” assay is an enzymatic assay which is much more specific for glycated serum protein (fructosamine) than the old-traditional NBT based fructosamine assay which is significantly interfered by endogenous reducing substances. NBT reacts with various endogenous substances of reducing activity such as thiol groups, NADH and ascorbate. In fact, studies have shown that only about half of the reducing activity (fructosamine) was due to specific non-enzymatic glycation of proteins, and the remaining unspecific activity varied from serum to serum.
Overview
Sample material
Use 20 µl fresh li-hep plasma or serum.
Samples can be stored at 2-8 °C for two weeks or at -20 °C for up to four weeks.
Method / Measurement range
Photometric measurement of fructosamine in serum or EDTA plasma at 546 nm wave length as enzymatic end point test.
0 – 2600 µmol/l (LOT dependent)
Test duration
Test duration takes approx. 9 minutes.
Package insert
More details can be found in the package insert.
Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.
Appropriate instruments
Evaluation
Test evaluation
Find the evaluation study for the Eurolyser fructosamine assay here: Eurolyser fructosamine test evaluation
Additional information
Article numbers
VT 0110 Fructosamin test kit (containing 16 tests)
VT 0111 Fructosamin test kit (containing 6 tests)
VT 1100 Fructosamin control kit (decision level)