Fructosamine is an ideal parameter for the monitoring of diabetic animals. Fructosamine arises by the binding of glucose to proteins. In dogs glucose has a stronger affinity to albumins, and globulins in cats. Because of the half-life time of plasma proteins a single measurement of fructosamine indicates the average glucose concentration over the previous 1 to 2 weeks.

Individual measurements of fructosamine should be interpreted in view of the clinical symptoms of diabetes, the body weight and the blood glucose concentration. The general rule is, the closer the fructosamine concentration to the reference range for healthy dogs and cats, the better the glycemic control.

Eurolyser’s “Gold standard fructosamine” assay is an enzymatic assay which is much more specific for glycated serum protein (fructosamine) than the old-traditional NBT based fructosamine assay which is significantly interfered by endogenous reducing substances. NBT reacts with various endogenous substances of reducing activity such as thiol groups, NADH and ascorbate. In fact, studies have shown that only about half of the reducing activity (fructosamine) was due to specific non-enzymatic glycation of proteins, and the remaining unspecific activity varied from serum to serum.

Overview

Sample material

Use 20 µl fresh li-hep plasma or serum.
Samples can be stored at 2-8 °C for two weeks or at -20 °C for up to four weeks.
 

Method / Measurement range

Photometric measurement of fructosamine in serum or EDTA plasma at 546 nm wave length as enzymatic end point test.

0 – 2600 µmol/l (LOT dependent)
 

Test duration

Test duration takes approx. 9 minutes.
 

Package insert

More details can be found in the package insert.

Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.

Appropriate instruments

Eurolyser CUBE-VET

Eurolyser solo

Evaluation

Test evaluation

Find the evaluation study for the Eurolyser fructosamine assay here: Eurolyser fructosamine test evaluation

Additional information

Article numbers

VT 0110      Fructosamin test kit (containing 16 tests)
VT 0111      Fructosamin test kit (containing 6 tests)
VT 1100      Fructosamin control kit (decision level)