Eurolyser Diagnostics - Premium Laboratory Equipment

T4 Test


About the parameter

Thyroxin (T4) is formed in thyroid and released into the bloodstream, passing a complex regulating mechanism. Thyroxin is bound to several carrier proteins such as albumin, pre-albumin and other specific binding proteins. Thyroid diseases with abnormal hormone concentrations mainly effect older animals.

Increased – or decreased, respectively – concentration levels of thyroid hormones such as Thyroxin (T4) lead towards numerous organic disorders. Thyroid hyperfunction effect mainly cats, whereas thyroid hypofunction affects all species.


Sample material

Li-hep plasma, EDTA plasma or serum. Sample volume according to the menu settings on Eurolyser solo instrument and according to the use of a 20 µl or 5 µl pipette.

Method / Measurement range

The Eurolyser solo T4 VET analysis is a homogenous enzyme immunoassay. By help of 8-anilino-1-naphtalene acid sulfur (ANS) the bound T4 is seperated from its natural binding proteins. The thyroxin seperated from blood competes with a thyroxin bound to a glucose-6-phosphat dehydrogenase to form a specific connector. GAPDH activity at 340 nm is associated with the total thyroxin concentration in blood. If T4 is absent in the blood sample the enzyme activity is inhibited by binding of a specific antibody.

Measurement range
When using a 20 µl pipette: 0.4 – 8 µg/dl (5.2 – 104 nmol/l), starting from LOT 2401-1: 0.4 – 20 µg/dl (5.2 – 260 nmol/l)
When using a 5 µl pipette: 6 – 32 µg/dl (78 – 416 nmol/l)

Test duration

This Test takes approx. 6 minutes.

Package insert

More details can be found in the package insert:
package insert
package insert (starting from LOT 2401-1)

Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.

Appropriate instruments

Eurolyser CUBE-VET

Eurolyser solo


Test evaluation

Find the evaluation study for the Eurolyser T4 assay here:
Eurolyser T4 test evaluation
Eurolyser T4 test evaluation (starting from LOT 2401-1)

Additional information

Article numbers

VT 0140      T4 test kit (containing 16 tests)
VT 0141      T4 test kit (containing 6 tests)
VT 1400      T4 control kit (decision level)

Eurolyser News

18. August 2020

SDMA: A new veterinary assay for evaluation of chronic kidney diseases...
»

17. August 2020

Phenobarbital: A next generation epilepsy monitoring assay...
»

10. December 2019

Launch of bidirectional connectivity: a feature for CUBE series instruments...
»

5. December 2019

Eurolyser is now ISO 13485:2016 certified...
»