T4 Test
Thyroxin
About the parameter
Thyroxin (T4) is formed in thyroid and released into the bloodstream, passing a complex regulating mechanism. Thyroxin is bound to several carrier proteins such as albumin, pre-albumin and other specific binding proteins. Thyroid diseases with abnormal hormone concentrations mainly effect older animals.
Increased – or decreased, respectively – concentration levels of thyroid hormones such as Thyroxin (T4) lead towards numerous organic disorders. Thyroid hyperfunction effect mainly cats, whereas thyroid hypofunction affects all species.
Overview
Sample material
Li-hep plasma, EDTA plasma or serum. Sample volume according to the menu settings on Eurolyser solo instrument and according to the use of a 20 µl or 5 µl pipette.
Method / Measurement range
The Eurolyser solo T4 VET analysis is a homogenous enzyme immunoassay. By help of 8-anilino-1-naphtalene acid sulfur (ANS) the bound T4 is seperated from its natural binding proteins. The thyroxin seperated from blood competes with a thyroxin bound to a glucose-6-phosphat dehydrogenase to form a specific connector. GAPDH activity at 340 nm is associated with the total thyroxin concentration in blood. If T4 is absent in the blood sample the enzyme activity is inhibited by binding of a specific antibody.
Measurement range
When using a 20 µl pipette: 0.4 – 8 µg/dl (5.2 – 104 nmol/l), starting from LOT 2401-1: 0.4 – 20 µg/dl (5.2 – 260 nmol/l)
When using a 5 µl pipette: 6 – 32 µg/dl (78 – 416 nmol/l)
Test duration
This Test takes approx. 6 minutes.
Package insert
More details can be found in the package insert:
package insert
package insert (starting from LOT 2401-1)
Please note: All package inserts provided on this website are intended for information purposes only! Always process all tests, controls and calibrators strictly according to the package insert provided with the kit.
Appropriate instruments
 Eurolyser CUBE-VET |
 Eurolyser solo |
Evaluation
Test evaluation
Find the evaluation study for the Eurolyser T4 assay here:
Eurolyser T4 test evaluation
Eurolyser T4 test evaluation (starting from LOT 2401-1)
Additional information
Article numbers
VT 0140 T4 test kit (containing 16 tests)
VT 0141 T4 test kit (containing 6 tests)
VT 1400 T4 control kit (decision level)